rabbit polyclonal anti mmp13 Search Results


mmp13 polyclonal antibody  (Bioss)
94
Bioss mmp13 polyclonal antibody
Mmp13 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp13 polyclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
mmp13 polyclonal antibody - by Bioz Stars, 2026-03
94/100 stars
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mmp13  (Cusabio)
91
Cusabio mmp13
Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and <t>MMP13</t> determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.
Mmp13, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp13/product/Cusabio
Average 91 stars, based on 1 article reviews
mmp13 - by Bioz Stars, 2026-03
91/100 stars
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anti-mmp-13  (US Biological Life Sciences)
90
US Biological Life Sciences anti-mmp-13
Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and <t>MMP13</t> determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.
Anti Mmp 13, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mmp-13/product/US Biological Life Sciences
Average 90 stars, based on 1 article reviews
anti-mmp-13 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and MMP13 determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and MMP13 determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.

Article Snippet: Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature.

Techniques: Over Expression, Plasmid Preparation, Western Blot, Transfection, Negative Control, Flow Cytometry, TUNEL Assay, Staining, Apoptosis Assay, Expressing, Quantitative RT-PCR

Figure 3. Specific effects of HMGB1 on chondrocyte apoptosis and cellular inflammation (A) HMGB1 knockdown was achieved in chondrocytes by transfecting with small interfering RNA targeting HMGB1 (si-HMGB1), which was confirmed using western blot analysis. Next, chondrocytes were transfected with si-HMGB1 or si-NC (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h). (B) Cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit. (C) The mRNA expressions of IL-6, TNF-α, INOS, and COX2 were examined by qRT-PCR. (D) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis. (E) The protein levels of p- p65 and p65 were examined by western blot analysis. *P<0.05, **P<0.01 vs si-NC group; ††P<0.01 vs si-NC+IL-1β group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 3. Specific effects of HMGB1 on chondrocyte apoptosis and cellular inflammation (A) HMGB1 knockdown was achieved in chondrocytes by transfecting with small interfering RNA targeting HMGB1 (si-HMGB1), which was confirmed using western blot analysis. Next, chondrocytes were transfected with si-HMGB1 or si-NC (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h). (B) Cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit. (C) The mRNA expressions of IL-6, TNF-α, INOS, and COX2 were examined by qRT-PCR. (D) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis. (E) The protein levels of p- p65 and p65 were examined by western blot analysis. *P<0.05, **P<0.01 vs si-NC group; ††P<0.01 vs si-NC+IL-1β group.

Article Snippet: Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature.

Techniques: Knockdown, Small Interfering RNA, Western Blot, Transfection, Negative Control, Flow Cytometry, Quantitative RT-PCR

Figure 4. Nrf2 acts on chondrocytes through the HMGB1/NFκB pathway (A) Chondrocytes were cotransfected with Nrf2 OE and HMGB1 OE and examined for the protein levels of Nrf2 and HMGB1 using western blot analysis. (B‒E) In the presence of IL-1β stimulation (10 ng/mL for 24 h), cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit (B); the mRNA expression of IL-6, TNF-α, INOS, and COX2 was examined by qRT-PCR (C); the protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis (D); the protein levels of p-p65 and p65 were examined by western blot analysis (E). *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+HMGB1 OE group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 4. Nrf2 acts on chondrocytes through the HMGB1/NFκB pathway (A) Chondrocytes were cotransfected with Nrf2 OE and HMGB1 OE and examined for the protein levels of Nrf2 and HMGB1 using western blot analysis. (B‒E) In the presence of IL-1β stimulation (10 ng/mL for 24 h), cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit (B); the mRNA expression of IL-6, TNF-α, INOS, and COX2 was examined by qRT-PCR (C); the protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis (D); the protein levels of p-p65 and p65 were examined by western blot analysis (E). *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+HMGB1 OE group.

Article Snippet: Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature.

Techniques: Western Blot, Flow Cytometry, Expressing, Quantitative RT-PCR, Plasmid Preparation

Figure 5. Effects of Nrf2 and HMGB1 on OA mouse damage in vivo Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) surgery, and mice were assigned into five groups: (i) Sham, (ii) DMM, (iii) DMM+TBHQ, (iv) DMM+rHMGB1 and (v) DMM+TBHQ+rHMGB1. (A) Histopathological alterations in knee articular cartilage from different groups of mice were verified by H&E staining, scale bar: 100 μm. (B) The expression levels of inflammatory factors (IL-6, TNF-α, iNOS, and COX2) in knee articular cartilage from different groups of mice were determined by ELISA. (C) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 in knee articular cartilage from different groups of mice were examined by western blot analysis. (D) The protein levels of p-p65 and p65 in knee articular cartilage from different groups of mice were examined by western blot analysis. *P<0.05, **P<0.01 vs sham group; ††P<0.01 vs DMM group; ##P<0.01 vs DMM+rHMGB1 group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 5. Effects of Nrf2 and HMGB1 on OA mouse damage in vivo Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) surgery, and mice were assigned into five groups: (i) Sham, (ii) DMM, (iii) DMM+TBHQ, (iv) DMM+rHMGB1 and (v) DMM+TBHQ+rHMGB1. (A) Histopathological alterations in knee articular cartilage from different groups of mice were verified by H&E staining, scale bar: 100 μm. (B) The expression levels of inflammatory factors (IL-6, TNF-α, iNOS, and COX2) in knee articular cartilage from different groups of mice were determined by ELISA. (C) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 in knee articular cartilage from different groups of mice were examined by western blot analysis. (D) The protein levels of p-p65 and p65 in knee articular cartilage from different groups of mice were examined by western blot analysis. *P<0.05, **P<0.01 vs sham group; ††P<0.01 vs DMM group; ##P<0.01 vs DMM+rHMGB1 group.

Article Snippet: Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature.

Techniques: In Vivo, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot